There is no one type which is “best” – it all depends on what your samples are and what sort of result you want to achieve.
Use the questions below to help you narrow down the options and choose the homogenizer which best suits your work.
Does the standard method or SOP define the type?
If you’re working to an established SOP or standardised method, or if you’re replicating a previously reported method, the same homogeniser that this recommends should be used to achieve comparable results.
What sample volumes are you working with?
What is the sample tissue?
Dry samples without any liquid are harder to process, but some (such as hair) can be homogenised in a bead ruptor.
For hard samples such as bone, cartilage or skin a bead ruptor is the best option.
What is the sample liquid?
You also need to consider the chemical compatibility of the buffer or solvent – bead ruptor units use polypropylene tubes, while some tips may be polycarbonate.
If you’re working with organic solvents a stainless steel rotor stator probe should be selected.
When working with volatile solvents, the work should be done inside a fume hood.
What end result is required?
To achieve a result with particles of 20 microns or larger a blade assembly should be chosen.
Is cross contamination a concern?
Stainless steel probes can be taken apart for cleaning, but then re-used – hence there is a risk of cross contamination dependent on the cleaning.
For more advice on choosing a homogeniser that’s right for your application, just contact us;