Posts tagged 'ethidium bromide'

How to detect Ethidium Bromide
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Cleaning a Laboratory: how to detect Ethidium Bromide

Ethidium Bromide  or "EtBr" is a fluorescent nucleic acid tag commonly used in molecular biology laboratories to detect DNA and RNA. When exposed to UV light EtBr will fluoresce with a orange/red colour, this fluorescence will intensify 20-fold once bound to DNA. Although no confirming scientific studies on the subject, Ethidium Bromide is feared to be toxic due to the way it insets itself in D...

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What gloves are resistant to ethidium bromide?

Ethidium bromide (Et/Br) is the most commonly used stain for detecting DNA/RNA. The reason for this is its sensitivity and yields a low background and detection limit. The image below shows bands in gel with EtBr and how they fluoresce under UV light. But the major drawback to EtBr is that the it is a potent mutagen and solutions must Be handled with extreme caution and decontaminated pr...

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